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Microarray Analysis is a natural outgrowth of the author's teaching and research into the field of microarray processing, a fast growing and developing field. The book highlights the development of various microarray analysis tools and the applications of the technology towards disease prevention and eradication. The goal is to illustrate the many sequencing methods in place, and to show the various applications of these methods. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fibrosis in children consanguineous marriages in Saudi Arabia, genetic disorders, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes, biochip manufacturing methods, advances in sequencing methods, microfluids, electrophoretic methods are discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule is explored. The criteria for choosing the best surface to use for microarray analysis is elloborated. Additionally, Microarray Analysis: Biochips and Eradication of Disease will feature the statistical methods needed for analysis of DNA data from microarray analysis, including shotgun and next generation sequencing.
DNA microarrays. --- DNA biochips --- Microarrays, DNA --- Biochips --- Immobilized nucleic acids
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Biochips. --- Bioreactors --- Molecular computers --- Combinatorial chemistry
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Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.
DNA microarrays. --- Gene expression. --- Genes --- Genetic regulation --- DNA biochips --- Microarrays, DNA --- Biochips --- Immobilized nucleic acids --- Expression
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DNA microarrays. --- DNA --- DNA biochips --- Microarrays, DNA --- Biochips --- Immobilized nucleic acids --- Deoxyribonucleic acid synthesis --- Synthesis.
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Biochips --- Cooperating objects (Computer systems) --- Testing. --- Automatic control. --- Security measures. --- Bioreactors --- Molecular computers --- Combinatorial chemistry
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538.9 --- 538.9 Physics of condensed matter (in liquid state and solid state) --- Physics of condensed matter (in liquid state and solid state) --- Fluidic devices --- Biochips --- Microfluidics --- Fluidics --- Nanofluids --- Fluid power technology --- Bioreactors --- Molecular computers --- Combinatorial chemistry --- Biochips. --- Fluidic devices. --- Microfluidics. --- Dispositifs fluidiques --- Microfluidique --- Biopuces
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Genetic toxicology. --- DNA microarrays --- Health risk assessment. --- Carcinogenesis. --- Statistical methods. --- Cancer --- Oncogenesis --- Pathogenesis of cancer --- Tumorigenesis --- Assessment, Health risk --- Health hazard appraisal --- Health hazard assessment --- Health risk appraisal --- HRA (Public health) --- Human risk assessment --- DNA biochips --- Microarrays, DNA --- Genotoxicity --- Genotoxicology --- Toxicology --- Pathogenesis --- Genetic aspects --- Pathology --- Genetic toxicology --- Medicine, Preventive --- Public health --- Risk assessment --- Environmental health --- Biochips --- Immobilized nucleic acids --- Biochemical genetics --- Medical genetics --- Carcinogenesis --- Chemical mutagenesis
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In recent years, a number of academic and commercial software packages and databases have been developed for the analysis and screening of biological data; however, the usability of these data is compromised by so-called novel genes to which no biological function is assigned. Annotating new genes outlines an approach to the analysis of evolutionary-conserved, heart-enriched genes with unknown functions, offering a step-by-step description of the procedure from screening to validation. The book begins by offering an introduction to the databases and software available, before moving on to cove
Bioinformatics. --- Biometry. --- Genetics. --- Genomics. --- Bioinformatics --- Genomics --- Computing Methodologies --- Databases, Factual --- Biology --- Databases as Topic --- Biological Science Disciplines --- Information Science --- Information Storage and Retrieval --- Information Systems --- Natural Science Disciplines --- Disciplines and Occupations --- Medical Informatics Applications --- Medical Informatics --- Databases, Genetic --- Computational Biology --- Software --- Health & Biological Sciences --- Genetics --- Biology - General --- Chemogenomics. --- Proteomics. --- Pharmacogenetics. --- Biochemical genetics. --- DNA microarrays. --- Combinatorial chemistry. --- Chemical genomics --- Genomes --- Biochemical genetics --- Chemicals --- Combinatorial organic synthesis --- Combinatorial synthesis (Organic chemistry) --- High-throughput organic synthesis --- High-throughput synthesis (Organic chemistry) --- Multiple parallel synthesis (Organic chemistry) --- Organic compounds --- Pharmaceutical chemistry --- Biochips --- DNA biochips --- Microarrays, DNA --- Immobilized nucleic acids --- Chemical genetics --- Biochemistry --- Molecular genetics --- Pharmacology --- Molecular biology --- Proteins --- Effect of chemicals on --- Physiological effect --- Synthesis --- Genetic aspects
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Chemistry, Pharmaceutical. --- Pharmaceutical Preparations --- Drug Design. --- Chemistry, Pharmaceutical --- Quimica farmaceutica --- Combinatorial chemistry. --- Chimie combinatoire --- chemical synthesis. --- 547.057 --- Organic synthesis --- Combinatorial chemistry --- Combinatorial organic synthesis --- Combinatorial synthesis (Organic chemistry) --- High-throughput organic synthesis --- High-throughput synthesis (Organic chemistry) --- Multiple parallel synthesis (Organic chemistry) --- Organic compounds --- Pharmaceutical chemistry --- Biochips --- Synthesis
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